文章题目：FattyLiverDiseaseCausedbyHigh-Alcohol-ProducingKlebsiellaPneumoniae
高产酒精的肺炎克雷伯菌可引起脂肪肝
文章出处：CellMetab.Oct01;30(4)；DOI:10./j.cmet..08.
首都儿科研究所、中科院武汉病*研究所、*事医学科学院微生物流行病研究所
SUMMARY
Theunderlyingetiologyofnonalcoholicfattyliverdisease(NAFLD)isbelievedtobequitevaried.Changesinthegutmicrobiotahavebeeninvestigatedandarebelievedtocontributetoatleastsomecasesofthedisease,thoughacausalrelationshipremainsunclear.Here,weshowthathighalcoholproducingKlebsiellapneumoniae(HiAlcKpn)isassociatedwithupto60%ofindividualswithNAFLDinaChinesecohort.TransferofclinicalisolatesofHiAlcKpnbyoralgavageintomiceinducedNAFLD.Likewise,fecalmicrobiotatransplant(FMT)intomiceusingaHiAlc-Kpn-strain-containingmicrobiotaisolatedfromanindividualwithNASHinducedNAFLD.However,selectiveeliminationoftheHiAlcKpnstrainbeforeFMTpreventedNAFLDintherecipientmice.TheseresultssuggestthatatleastinsomecasesofNAFLDanalterationinthegutmicrobiomedrivestheconditionduetoexcessendogenousalcoholproduction.
Methods
BacterialStrainsandGrowthConditions.Fecalsamplesofthepatientcloselyforoneincidencecyclewerecollected,cultivatedandpurifiedinbothBH(BrainHeart)andYPDMedium(withoutorwith5%alcoholasselectivemedium)underanaerobicandaerobicconditionsat37Cfor24h.AnaerobicconditionwasachievedinjarsusingAnaeroPacks(MitsubishiGasChemicalCompany,Tokyo,Japan).StandardstrainK.pneumoniaeATCCwasusedascontrol.
主要内容：非酒精性脂肪肝（NAFLD）是指除酒精等明确肝损伤因素外其他因素所导致的代谢应激性肝损伤，其致病因素至今仍不明确，近年研究表明这种疾病与肠道菌群的改变有密切关联。首都儿科研究所袁静、中科院武汉病*研究所刘翟、北京微生物流行病研究所杨瑞馥与团队在CellMetabolism发表的最新研究发现，在NAFLD临床队列中发现病人肠道内HiAlcKpn普遍存在（61%）。研究人员成功建立了菌群诱发的NAFLD小鼠模型，通过无菌小鼠、粪菌移植、噬菌体和抗生素治疗，以及浸润细胞特征性信号分子分析，证明了肠道菌群中HiAlcKpn是NAFLD肝病发生发展的新病因，高产乙醇的HiAlcKpn在肠道内定殖产生大量乙醇，经门脉系统到肝脏内，引起肝细胞线粒体的功能失调而形成脂肪肝，这在高产酒精的肠道菌群引起非酒精性脂肪肝致病机制领域取得重要进展
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